How to Verify a Peptide COA — Janoshik Verification Guide
QSC Research Guide
How to Verify a Peptide COA — Janoshik Verification Guide
QSC publishes a Janoshik-independently verified Certificate of Analysis for every production batch. This guide explains how to read a COA, interpret HPLC data, and use the Janoshik portal to independently confirm QSC’s testing results.
Why COA Verification Matters
The research peptide market contains products of vastly different quality — from ≥99% HPLC-pure compounds to mislabelled, under-dosed, or adulterated compounds. A COA from an independent third party is the only way to distinguish these without your own analytical equipment. QSC addresses this with Janoshik independent verification — every batch is tested by Janoshik and the result is publicly searchable.
What a QSC COA Contains
Batch number
Unique identifier linking this COA to a specific production batch. Used to look up results on Janoshik.
HPLC purity %
The main analytical measurement — peak area analysis showing what % of the sample is the target compound.
HPLC chromatogram
The actual graph of separated compounds. The main peak = your compound. Minor peaks = impurities.
Mass spectrometry (MS) result
Confirms the molecular weight matches the target compound. Essential for identity confirmation beyond purity.
Janoshik reference
The verification code for lookup at verify.janoshik.com.
How to Read an HPLC Chromatogram
The main peak
The tallest peak in the chromatogram is your target compound. Its retention time (x-axis) should match the reference for that compound.
Peak area %
Peak area ÷ total area = purity. QSC compounds show ≥99% main peak area. A chromatogram with one dominant peak and very small or absent minor peaks indicates high purity.
Minor peaks (impurities)
Small peaks adjacent to the main peak are impurities — typically deletion sequences (peptides missing one amino acid) or oxidation products. At ≥99% purity, these should account for <1% total area.
What to be suspicious of
An HPLC peak that is very broad (indicating multiple co-eluting compounds), multiple major peaks of similar height, or no published chromatogram at all — all indicate potential quality issues.
How to Verify via Janoshik
Step 1 — Locate the batch reference
Find the Janoshik batch reference number on the QSC COA or product page. It typically appears as a J-prefix alphanumeric code.
Step 2 — Visit verify.janoshik.com
Navigate to https://janoshik.com/verification/ in your browser.
Step 3 — Enter the batch reference
Enter the batch reference in the search field and submit. Janoshik returns the independently confirmed purity and identity results for that batch.
Step 4 — Compare with the QSC COA
The Janoshik result should match the QSC COA. Matching results confirm the COA is genuine and was produced by independent third-party analysis — not self-reported.
QSC vs. Self-Tested COAs
Testing performed by
Janoshik (independent laboratory) — not QSC
Result verifiable by anyone?
Yes — via verify.janoshik.com
Can result be fabricated?
No — Janoshik results are stored independently. QSC cannot modify or fake Janoshik records.
Batch-level or blanket?
Per-batch — every individual production batch has its own Janoshik COA
Self-tested COA alternative
Cannot be independently verified — researcher must trust supplier’s claim
Frequently Asked Questions
What is a COA and why does it matter?
A Certificate of Analysis confirms a compound’s purity and identity. A legitimate COA includes HPLC purity % and mass spectrometry molecular weight confirmation. Without MS, purity data alone cannot confirm you have the right compound — only that something is pure.
What is Janoshik and how do I verify a QSC COA?
Janoshik is an independent analytical laboratory. QSC submits every production batch to Janoshik for third-party testing. Results are stored independently and publicly searchable at verify.janoshik.com using the batch reference on the QSC COA.
What should I look for in an HPLC chromatogram?
One dominant main peak representing ≥99% of total peak area. Very small or absent minor peaks. The main peak retention time matching the reference for that compound. A broad main peak or multiple significant peaks indicates impurity.
Why do some suppliers not use Janoshik?
Third-party testing costs money and cannot be faked. Suppliers without it rely on self-reported COAs which cannot be independently confirmed. QSC’s Janoshik verification provides publicly confirmable proof of testing that self-tested COAs fundamentally cannot match.
Research Use Only: All QSC compounds are sold strictly for laboratory research purposes only. Not for human consumption, veterinary use, or any other application. Researchers are responsible for compliance with local regulations.
QSC Network
Compound reference, COA verification, and regional warehouse information across the QSC network.
