LKKTET motif binds G-actin monomers β promotes actin polymerisation at wound margins β cell migration for healing
ANTI-INFLAMMATORY
Downregulates NF-ΞΊB inflammatory pathway, reduces inflammatory cytokine production β broader anti-inflammatory effect than BPC-157
ANGIOGENESIS
Promotes endothelial cell migration via actin dynamics β new capillary formation in wound bed β systemic distribution advantage
Research Questions Answered
What is TB-500?
TB-500 is a synthetic peptide fragment corresponding to the active region (amino acids 17β23) of Thymosin Beta-4 (TΞ²4) β a naturally occurring 43-amino acid peptide ubiquitous in mammalian tissues. The active heptapeptide sequence Ac-LKKTETQ is responsible for most of TΞ²4’s tissue-repair activity. TB-500 sequesters G-actin (free actin monomers), modulates actin polymerisation dynamics, and promotes cell migration, angiogenesis, and anti-inflammatory signalling.
How does TB-500 work?
TB-500 works through the LKKTET actin-binding motif: it sequesters G-actin monomers, regulating the G-actin/F-actin equilibrium and promoting actin treadmilling at wound margins. This enables faster cell migration (fibroblasts, endothelial cells, macrophages) to injury sites. Additionally: TB-500 downregulates NF-ΞΊB inflammatory signalling, promotes angiogenesis by facilitating endothelial cell migration, and has been shown to modulate stem cell homing to injured tissues.
What is the difference between TB-500 and BPC-157?
TB-500 and BPC-157 are complementary tissue repair peptides with distinct mechanisms: TB-500 (7 AA): actin sequestration mechanism β primarily regulates cell migration, has stronger anti-inflammatory profile, more systemic distribution, effective for muscle tears and systemic injuries. BPC-157 (15 AA): NOS/VEGF/EGF mechanism β primarily promotes angiogenesis and tendon/GI repair, more localised tissue effect. Together they cover different aspects of tissue repair β frequently researched in combination.
What is TB-500 used for in research?
TB-500 research applications: muscle repair models (skeletal muscle tear, crush injury), cardiac tissue protection (Thymosin Ξ²4 has Phase 2 cardiac data for acute MI), tendon and ligament repair, wound healing (skin and soft tissue), angiogenesis research, anti-inflammatory models, and stem cell homing research. TB-500’s broader anti-inflammatory mechanism makes it useful across a wider tissue range than BPC-157.
Where can I buy TB-500 for research?
Research-grade TB-500 is available from QSC (qscpeptide.com) at β₯99% HPLC purity with Janoshik COA. US Domestic Warehouse: 2β4 business day delivery. Available in 10mg vial configurations. All products sold strictly for in vitro laboratory research only.
Does Thymosin Beta-4 have clinical trial data?
Full-length Thymosin Beta-4 (not TB-500 fragment) has clinical trial data: RegeneRx’s Phase 2 trials in dry eye disease and cardiac surgery showed safety and preliminary efficacy. The cardiac data (CHEETAH trial) showed some positive trends in MI recovery. TB-500 is the research fragment form β the active region. Full TΞ²4 clinical development is ongoing.
What is TB-500 molecular weight?
TB-500 has a molecular weight of approximately 902.1 Da. The sequence is Ac-Lys-Lys-Thr-Glu-Thr-Gln (Ac-LKKTETQ, 7 amino acids with N-terminal acetylation). The acetyl group is important for activity β it stabilises the N-terminus and improves binding to G-actin.
How is TB-500 stored and reconstituted?
Lyophilised TB-500: store at β20Β°C. Reconstituted: store at 4Β°C, use within 28 days. Reconstitute with bacteriostatic water (add slowly down vial wall, swirl gently). Standard: 2ml BW per 10mg vial = 5mg/ml. TB-500 is stable as a lyophilised powder for 24+ months at β20Β°C.
Is TB-500 the same as Thymosin Beta-4?
No β TB-500 is the 7-amino acid fragment (Ac-LKKTETQ, positions 17β23) of the full 43-amino acid Thymosin Beta-4 protein. TB-500 contains the actin-binding LKKTET motif which is responsible for most of TΞ²4’s activity. Research using TB-500 studies this specific active region, while full TΞ²4 research examines the complete protein including additional binding domains. For most tissue repair research applications, TB-500 produces comparable effects to full TΞ²4.
What purity should research-grade TB-500 be?
Research-grade TB-500 should be β₯99% HPLC purity with mass spectrometry confirmation. The acetylation of the N-terminus is critical β MS analysis should confirm correct acetylation along with sequence identity. QSC provides Janoshik-verified COAs for all TB-500 batches, verifiable at verify.janoshik.com.
Where to Buy Research-Grade TB-500
β₯99% HPLC Β· Janoshik COA Β· US Domestic 2β4 Days Β· Research Use Only
